Hi Paul,
Thanks for the compliments. If it helps, these are the details of the photo:
Camera: Nikon Coolpix 4500.
Focusing Distance: 2.5 cm.
f number: ~10 (and the optical zoom was at about 50%, the so-called "sweet spot" for macro).
Lighting: The in-camera flash was used but not to light the subject (the embryos were far too close to the camera lens). Instead, a large external speedlight/flash with dual output (small front speedlight as well as the main output) was used to light the subject, triggered via remote slave operation using the camera's flash. The speedlight provides ambient lighting using bounced light from its main output and direct lighting from the front small flash (above, slightly behind the camera at about 60<sup>o</sup> from the horizontal axis to the left - the camera is facing the subject from the right rather than directly facing the glass in order to minimise reflections). The output level of the speedlight was tuned until the photos looked about right (one of the great advantages of digital as I'm sure you know).
The in-camera settings were ISO 100, low contrast, no sharpening (I do sharpening on the computer as it's far superior to the camera's algorithms) and +0.7 exposure compensation (necessary for the 4500 due to some quirk from the low contrast setting).
Best regards,
John