Green fluorescent protein (GFP) Expression in month old Larvae

[usafaux2004]

I've noticed that Tank the Peg has a greenish hue to him. Not being sure if he's a WIldlite or a Leucistic with speckles (given the albino differences I'm seeing now, probably a Leucistic), I've definitely been observing him more than others. He's slowly been turning a yellowish/green color, but not a wildtype color, just a hue to him. Since both parents are GFP, I figured he has the trait as well.

Well, the results are in, and the looks are kinda "once you see it' type of deal.

Three of the Wildlites/Leucistics:

You can clearly see which two are GFP based on their color differences from the other.

Same three, different angle:

Three wildtypes, with two facing left being GFP:

Wildtype and Golden Albino "Twins". The Wildtype has glowing splotches, but nothing major. The Golden is quite a different story:

The same pair from above + Tank the Peg, who is a WIldlite/Leucistic:

Tank is quite the strobe light:

Bonus feature: Spot the Wildtype being a hunter.
Spot is the axie with a weird white eye and a dark one (the center instead of being black seems to leak light, and from above the eye looks oval due to a spot). He was the first to discover the 3rd Dimension, and is the only one I've been able to get to follow "prey" while swimming. Naturally one time the pray became my finger, so one time we played for a few minutes with him swimming back and forth after my pinkie. Now every time someone walks up to the containers, he swims up expecting someone to play (or to feed him, you know.)


VIDEO ON YOUTUBE:
http://youtu.be/itbAWAAUCSc

Can't seem to imbed the link...

 

[Kaysie]

Leucistics, indeed.

If both parents are GFP, all of your offspring will be as well.

 

[usafaux2004]

It's not recessive? Not all glow, 19/26.

 

[Kaysie]

As far as I understand, it's dominant. I wonder if your handful that don't will express any as they grow.

 

[usafaux2004]

Read on here a theory that maybe het or hom may affect expression. Maybe one parent is G/g and other is G/G, leading most to be G/G and some to G/g, accounting for less glow. The wilds glow little as is, but I have leucistics and albinos that simply don't.

 

[Kaysie]

Are the GFP cells especially abundant on/in any of the internal organs? Just out of curiosity.

 

[usafaux2004]

Ok, did a quick test on a black surface again. Wilds really only show it on lighter spots, so the black bottom helps see it easier.

7/9 Wilds are GFP
6/9 Leucys are GFP
6/8 Albinos are GFP

So 19/26, with no noticeable expression in 7 at all.

 

[oceanblue]

If both parents are GFP, all of your offspring will be as well.

My understanding is that since GFP manifests as a dominant both parents may have a chromosome which does not carry the GFP construct, so non GFP offspring may occur in up to 25% of some broods in these circumstances.

7 out of 26 =26.9% good enough for Mendel!

 

[usafaux2004]

Here they are in their tanks:

Second tank on right, top left and center - two Leucys of different color due to GFP.

Here's my 5 gilled Albino:

Leucistic with some spots still there:

Spot the Wildtype with the weird eye:

What I'm sure is my golden (but also GFP) albino, a regular (non GFP) albino and a GFP Wildtype.